Check for error codes first by bridging the two ports in the diagnostic plug and checking the flashing check engine light. It will tell you if there is a fault or a disconnected sender.
You can google to get the instructions for it.
Check the wire is connected to the gauge temp sender
Check the sender works (remove it and put it in water with a thermometer, raise the temperature and use a multimeter to check the values it gives)
If you've changed anything at the back of the gauge cluster, check those connections are all ok.
I guess the gauge itself could fail too but I'd say it's pretty unlikely.
This might help -
http://mr2.neogruts.com/engine/engine.htm They'd be my steps to test.